apoa1 mimetic peptide (22a)  (GenScript corporation)

Journal: ACS Chemical Biology

Article Title: Nanodiscoidal Nucleic Acids for Gene Regulation

doi: 10.1021/acschembio.3c00038

Figure Lengend Snippet: Assembly and synthesis of nanodiscoidal nucleic acids (NNAs). (a) Scheme depicting the assembly of NNAs. The ND scaffold is assembled by preparing 80 nm small unilamellar vesicles (SUVs) and combining them with a modified ApoA1 mimetic peptide containing a Cys amino acid insertion. The NNA is generated by conjugating maleimide-linked DNA to the exposed thiols on the surface (lipid) and edge (peptide) of the scaffold. (b) Table of lipids used in the assembly of NDs and NNAs. DMPC is the majority component present in the NDs and the thiol lipid, Ptd-thioethanol (blue), is added to certain discs to prepare NNAs. (c) Table of ApoA1 mimetic peptides screened for the formation of NNAs. The original mimetic peptide, denoted as A, does not contain any Cys residues and was further modified in versions B–D at the N- and/or C-terminus. (d) ND scaffolds generated from the peptide screen as shown in (b). Excluding peptide A, each peptide was used to prepare two different versions of DNA conjugates, one with DMPC exclusively and referred to as NDs and a second type which included thiolated phospholipids and denoted as the NNA.

Article Snippet: The modified and unmodified 22A ApoA1 mimetic peptides ( Figure b) were purchased from Genscript (Piscataway, NJ, USA) with N- and C- termini capped with acetylation and amidation, respectively, and TFA salts were removed.

Techniques: Modification, Generated